c2c12 mouse adherent myoblasts  (BioResource International Inc)

Journal: Analytical Chemistry

Article Title: Simultaneous Measurement of Individual Mitochondrial Membrane Potential and Electrophoretic Mobility by Capillary Electrophoresis

doi: 10.1021/ac403849x

Figure Lengend Snippet: Reproducibility in multiple CE-LIF runs of mitochondria isolated from C2C12 cells. (A) Distribution of red/green ratios from three combined replicate runs, n = 950 detected events. (B) Q–Q plot of red/green ratios from individual runs vs combined data. (C) Distribution of corrected electrophoretic mobility from the three combined runs. (D) Q–Q plot of corrected mobility from individual runs vs combined data. See Figure S-2-3 of the for the distributions of individual runs, Figures S-2-4 and S-2-5 of the for data from depolarized controls, and Table S-2-1 for the normalized ss res for Q–Q plots of the . CE-LIF conditions as described in Figure .

Article Snippet: Adherent C2C12 mouse myoblast and L6 rat myoblast cells (ATCC, Manassas, VA) were cultured in vented 75 cm 2 flasks at 37 °C with 5% CO 2 in DMEM supplemented with 10% FBS.

Techniques: Isolation

Journal: Analytical Chemistry

Article Title: Simultaneous Measurement of Individual Mitochondrial Membrane Potential and Electrophoretic Mobility by Capillary Electrophoresis

doi: 10.1021/ac403849x

Figure Lengend Snippet: Comparison of polarized vs depolarized ROIs using mitochondria isolated from C2C12 cells. (A) Histograms of red/green ratio distributions of polarized ROI from samples and depolarized ROI from depolarized controls. Polarized ROI: n = 501 events, 3 runs. Depolarized ROI: n = 503 events, 3 runs. (B) Q–Q plot of red/green ratio comparing overall distributions of depolarized controls vs samples (◆) and depolarized vs polarized ROIs (○). (C) Histograms of corrected electrophoretic mobility distributions of polarized ROI from samples and depolarized ROI from depolarized controls. (D) Q–Q plot of corrected electrophoretic mobility comparing overall distributions of depolarized controls vs samples (◆) and depolarized vs polarized ROIs (○). See Figures S-2-8 and S-2-9 of the for the histograms of the overall distributions and Table S-2-1 for the normalized ss res for Q–Q plots of the . CE-LIF conditions are the same as in Figure .

Article Snippet: Adherent C2C12 mouse myoblast and L6 rat myoblast cells (ATCC, Manassas, VA) were cultured in vented 75 cm 2 flasks at 37 °C with 5% CO 2 in DMEM supplemented with 10% FBS.

Techniques: Comparison, Isolation

Journal: Mediators of Inflammation

Article Title: Elevation of IL-6 and IL-33 Levels in Serum Associated with Lung Fibrosis and Skeletal Muscle Wasting in a Bleomycin-Induced Lung Injury Mouse Model

doi: 10.1155/2019/7947596

Figure Lengend Snippet: IL-6 and IL-33 may synergistically cause muscle atrophy. (a) The quadriceps muscle of the mice was homogenized, and the lysate was analyzed by Western blotting with specific antibodies against STAT3, AMPK, and Atrogin-1. (b) C2C12 cells, mouse adherent myoblasts, were incubated with 2% horse serum for 72 hours and stimulated with recombinant mouse IL-6 and IL-33 in serum-free medium as indicated for 24 hours. The remaining cells were harvested, and the levels of p-STAT3, STAT3, p-AMPK α , and AMPK α in the cell lysate were analyzed by Western blotting. α -Tubulin and GAPDH were used as internal controls. The intensity of bands in the Western blots was measured by ImageJ software. The quantitative data were expressed as the means ± SD. ∗ p < 0.05 compared with control.

Article Snippet: C2C12 mouse adherent myoblasts were obtained from BCRC (Bioresource Collection and Research Center, Hsinchu, Taiwan).

Techniques: Western Blot, Incubation, Recombinant, Software, Control